t25 polystyrene cell culture flasks with cell growth surface for adherent cells (Sarstedt)
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Sarstedt
t25 polystyrene cell culture flasks with cell growth surface for adherent cells
T25 Polystyrene Cell Culture Flasks With Cell Growth Surface For Adherent Cells, supplied by Sarstedt, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polystyrene+cell+culture+flasks+t25/t25+polystyrene+cell+culture+flasks+with+cell+growth+surface+for+adherent+cells/pmc09719844-325-27-29
Average 90 stars, based on 1 article reviews
T25 Polystyrene Cell Culture Flasks With Cell Growth Surface For Adherent Cells, supplied by Sarstedt, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polystyrene+cell+culture+flasks+t25/t25+polystyrene+cell+culture+flasks+with+cell+growth+surface+for+adherent+cells/pmc09719844-325-27-29
Average 90 stars, based on 1 article reviews
t25 polystyrene cell culture flasks with cell growth surface for adherent cells - by Bioz Stars,
2026-10
90/100 stars
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Isolation:Article Title: Human adipose tissue-derived stem cells cultured in xeno-free culture condition enhance c-MYC expression increasing proliferation but bypassing spontaneous cell transformation Article Snippet: Briefly, 15 mL of the raw lipoaspirates were washed with phosphate-buffered saline (PBS) and enzymatically digested with 0.075% collagenase type I (Life Technologies, USA) in PBS at 37°C for 1 hour. .. Subsequently, the stromal vascular fraction was isolated by centrifugation at 252 g for 10 minutes, and the pellet was resuspended in basal medium, plated into Centrifugation:Article Title: Human adipose tissue-derived stem cells cultured in xeno-free culture condition enhance c-MYC expression increasing proliferation but bypassing spontaneous cell transformation Article Snippet: Briefly, 15 mL of the raw lipoaspirates were washed with phosphate-buffered saline (PBS) and enzymatically digested with 0.075% collagenase type I (Life Technologies, USA) in PBS at 37°C for 1 hour. .. Subsequently, the stromal vascular fraction was isolated by centrifugation at 252 g for 10 minutes, and the pellet was resuspended in basal medium, plated into Cell Culture:Article Title: Human adipose tissue-derived stem cells cultured in xeno-free culture condition enhance c-MYC expression increasing proliferation but bypassing spontaneous cell transformation Article Snippet: Briefly, 15 mL of the raw lipoaspirates were washed with phosphate-buffered saline (PBS) and enzymatically digested with 0.075% collagenase type I (Life Technologies, USA) in PBS at 37°C for 1 hour. .. Subsequently, the stromal vascular fraction was isolated by centrifugation at 252 g for 10 minutes, and the pellet was resuspended in basal medium, plated into Incubation:Article Title: Human adipose tissue-derived stem cells cultured in xeno-free culture condition enhance c-MYC expression increasing proliferation but bypassing spontaneous cell transformation Article Snippet: Briefly, 15 mL of the raw lipoaspirates were washed with phosphate-buffered saline (PBS) and enzymatically digested with 0.075% collagenase type I (Life Technologies, USA) in PBS at 37°C for 1 hour. .. Subsequently, the stromal vascular fraction was isolated by centrifugation at 252 g for 10 minutes, and the pellet was resuspended in basal medium, plated into |
![( A ) Milk MECs postisolation (P0) stained with rhodamine B after 12 days of culture . Milk MECs form colonies of different sizes and cell shapes, as shown with bright-field imaging. ( B ) Milk MECs can be expanded over several passages ( x axis, number of passages; y axis, cumulative cell number); means ± SD, n = 4 biological replicates represented in black, n = 2 or 1 biological replicate represented in gray. ( C ) Overlay of representative flow cytometry plot showing the cells postisolation in pink [epithelial cell adhesion marker negative (EpCAM − ) and integrin alpha-6 negative (CD49f − )] and during in vitro culture in cyan, demonstrating a shift toward EpCAM − /CD49 + (basal-like), EpCAM + /CD49f + (luminal progenitor), and EpCAM + /CD49f − (mature luminal) epithelial cell populations. ( D ) Distribution of MEC populations during isolation and through successive passages (P0 to P3). The color-coded legend denotes the following: pink: all negative (EpCAM − CD49f − ); dark blue: luminal progenitors (EpCAM + CD49f + ); light blue: luminal mature cells (EpCAM + CD49f − ); black: basal-like cells (EpCAM − CD49f + ). Data are represented as the means of four different donors. ( E ) Western blot showing the presence of prolactin (PRL) and PRLR in the milk MECs over three passages. GAPDH, glyceraldehyde-3-phosphate dehydrogenase. ( F ) Western blot showing the presence of FOXA1-expressing cells in two of the four donors at P0. ( G ) Immunofluorescent staining of milk MECs in 2D in vitro culture on <t>Matrigel-coated</t> <t>polystyrene</t> cell culture flasks with epithelial markers (EpCAM and CD49f), luminal marker (CK8), markers of proliferation (Ki67) and basal myoepithelial cells (P63) , and markers for tight junction formation (E-cadherin and ZO-1) and proliferation (Ki67). White arrowheads indicate binucleated cells. Scale bars, 50 μm. DAPI, 4′,6-diamidino-2-phenylindole.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_6022/pmc12136022/pmc12136022__sciadv.adu5793-f2.jpg)